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KMID : 0381120080300030191
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Genes and Genomics
2008 Volume.30 No. 3 p.191 ~ p.196
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Identification of AFLP and SCAR Markers Linked to the Fertility Restorer Gene for the ‘Polima’ CMS in Brassica napus
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Zhao Zhenqing
Liu Pingwu
Yang Guangsheng
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Abstract
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Polima (Pol) system of cytoplasmic male sterility (CMS) has been widely used for hybrid breeding in Brassica napus L in China. Molecular markers tightly linked to the restorer gene would be not only a valuable tool for breeding new restorer lines but also important landmarks for cloning the gene based on the linkage map. In order to identify DNA markers linked to its restorer gene (Rfp ), a near-isogenic line (NIL) population segregating for male sterility (rfprfp ) /fertility (Rfprfp ) was
subjected to amplified fragment length polymorphism (AFLP) analysis using the bulked segregant analysis (BSA) method. From the survey of 2,560 primer combinations, nine AFLP markers tightly linked to the target gene were identified. The distance between the two closest markers (ZQA and ZQH) and the target locus were both 0.4 cM, but they located at the same side of the Rfp locus. To exploit fast, cheap and reliable PCR-based markers for large-scale application in marker-assisted selection (MAS), polymorphic fragments of these two AFLP markers were sequenced and successfully converted into sequence characterized amplified region (SCAR) markers. This suite of markers could be valuable in developing new elite restorers in rapeseed.
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KEYWORD
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Brassica napus, cytoplasmic male sterility, fertility restorer gene, AFLP, SCAR, genetic mapping, MAS
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